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Meigs JB, Rutter MK, Sullivan LM, Fox CS, DAgostino RB, Wilson PWF

Cell culture transfection assays Transient cell transfection was carried out with TransIT-X2 Transfection Reagent (Mirus Bio, Madison, WI, USA) with plasmid constructs containing sequences encoding proIGF-1s as previously described 7

A 1mg dose delivers the same amount of GHK-Cu whether drawn from 10mg/ml solution (0.1ml) or 20mg/ml solution (0.05ml)

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